Curcumin-induced apoptosis in 3T3L1 cells
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Figure 5a.- Curcumin (40 µM) treatment showed clear decrease in the expression of PKA
catalytic subunit with time increasing in 3T3L1 cells compared with control.
Figure 5b.- Curcumin (40 µM) treatment showed an inhibiting effect on PKA activity (%)
during 4-24h on 3T3L1 cells.
In this study, FACS analysis showed that curcumin arrested the cell cycle.
Curcumin was shown to induce sequential G1/S and G2/M phase arrest in a
human osteosarcoma cell line (37) and S phase arrest in human colorectal
carcinoma cells (24).Our data demonstrated found that Curcumin induced
apoptosis and inhibited ROS generation level induced by the oxidant H2O2 in
3T3L1 adipocytes. This result could be explained by the findings of
Balasubramanyam et al (40) who showed that the antioxidant effects of curcumin
occurred via inhibition of Ca2+ influx and protein kinase C. In contrast,
Thayyullathil et al (41) reported that curcumin induced ROS generation, which in
turn resulted in apoptosis via activating apoptotic-inducing factor in mouse
