Curcumin-induced apoptosis in 3T3L1 cells
727
Figure 3.- Curcumin (40 μM) induces apoptosis (the number of cell death (%)) with time
increasing.
3.5. Curcumin-inhibits oxidative stress-induced apoptosis.
ROS relative fluorescence was measured after curcumin treatment (40 μM)
showed an inhibitory effect on ROS generation level with/without H2O2 treatment
of 3T3L1 adipocytes (Figure 6)
.
4. DISCUSION
This present study was performed to address contrasting data
demonstrating that Curcumin inhibits adipogenesis in 3T3-L1 adipocytes versus
data demonstrating that Curcumin activates proliferation of 3T3-L1 adipocytes. In
addition, the present studies aim to elucidate the underlying molecular mechanism
utilized by Curcumin. The results showed that increasing doses of curcumin
significantly inhibited 3T3L1adipocyte viability%. A lot of compelling evidences
have also shown that curcumin exerts an angio-inhibitory action by decreasing
vascular endothelial growth factor levels of NIH3T3 cells (17). These results were
in agreement with previous finding that curcumin inhibited differentiation and
increased apoptosis in 3T3-L1 (16) and in T -cell lymphoma cell lines (20).
Curcumin can significantly decrease leptin release in LPS-treated adipocytes in
