Curcumin-induced apoptosis in 3T3L1 cells
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Figure 1.- Viability % inhibition effect of different concentrations of curcumin ( 5- 80 µM ) on
3T3L1 adipocytes. Cells were incubated with curcumin for 24h. Viability of the cells
calculated by formula sample/control) x100.
Post-hoc testing (LSD) showed that the most significant inhibition (P ≤ 0.01)
started from the dose of 40 µM, which is physiologically relevant. Moreover,
induction of maximum inhibition by curcumin - as previously recommended- at (P
≤ 0.01) with this concentration (Figure 1). The selected dose (36- 37) showed a
clear gradual inhibition in 3T3L1 cell viability% in a time-dependent manner
(Figure 2), indicating that cell viability was significantly decreased (P ≤ 0.01) with
longer incubations (97.13% ± 0.85%, 90.42% ± 1.44%, 74.38% ± 1.87%,and
64.12% ± 3.54% at 4, 8, 12, and 24 h, respectively).
3.2. Curcumin induces apoptosis.
FACS analysis provided information about the extent of cell death (%). The
results clearly showed that curcumin (40 μM) induced apoptosis in a time-
dependent manner, indicated an increase in cell death % compared with control
(Figure 3).
