The roleof the catecholaminergic…
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Figure 6. Expression of receptors in the chick embryonic heart regions.
A) Analysis of
adrenergic receptors
(
α
-‐1b
,
α
-‐1d
and
α
-‐2c
;
β
-‐1
and
β
-‐2
) expression by RT-‐PCR of mRNA from the
precardiogenic area (stage 5), endocardial tubes (stage 8) or heart tube (stage 10). An internal
control with
GAPDH
is shown below. B) Analysis of dopaminergic receptors (D1, D3 and D5)
expressionby quantitative PCRofmRNA fromstage 10 and12heart tubes. The levels of dopamine
receptor transcriptswere normalized relative to
GAPDHmRNA
levels. Primers are shown inTables
1and2.
