C. Hernández-‐Sánchez, P. Vázquez, FloradePablo
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Figure 2. Novel forms of gene regulation in the
th-‐ins
locus.
A) The schematic genomic
organization of the chicken
th-‐ins
locus (there is a similar cluster in mammals although the
transcriptional regulationmaybedifferent) anddiagramof the transcripts generatedby theout-‐of-‐
frame splicing between
th
exon 13 and exon 2 or 3 of the insulin gene. B) TH isoforms generated
and their enzymatic activity was analyzed by HPLC for L-‐DOPA production. The extracts of
transfected HEK293T cells with these TH isoforms show a lower enzymatic activity than the
canonical TH. (Modified from[8])
B
